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HPLC with chemiluminescence detection of serum levels of precolumn derivatized fluoropyrimidine compounds
Summary
Type:
HPLC
Application #:
107102
Catalog#:
720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection:
fluorescence, excitation 346nm, emission 395nm
Conditions:
eluent: methanol - acetonitrile - water (29:5:66, v/v)
flow rate 1.0ml/min
Authors:
Yoshida Sh
Source:
J Chromatogr 383 (1986) 61 - 68
Details
Legend: Fig. 7. Chromatograms of A) serum blank, B) serum spiked with 10µg each of FdUrd and CldUrd, 4-bromomethyl-7-methoxycoumarin derivatives of 1. inosine, 2. uridine, 3. uracil, 4. thymine, 5. FdUrd, 6
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